Browsing by Author "Fayed, Marwa A. A"

Filter results by typing the first few letters
Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Chemical profling and cytotoxic potential of the n-butanol fraction of Tamarix nilotica fowers
    (BioMed Central Ltd., 2023-05) Fayed, Marwa A. A; Bakr, Riham O; Yosri, Nermeen; Khalifa, Shaden A. M; El‑Seedi, Hesham R; Hamdan, Dalia I; Refaey, Mohamed S; El‑Seedi, Hesham R; Hamdan, Dalia I; Refaey, Mohamed S
    Background Cancer represents one of the biggest healthcare issues confronting humans and one of the big chal‑ lenges for scientists in trials to dig into our nature for new remedies or to develop old ones with fewer side efects. Halophytes are widely distributed worldwide in areas of harsh conditions in dunes, and inland deserts, where, to cope with those conditions they synthesize important secondary metabolites highly valued in the medical feld. Several Tamarix species are halophytic including T.nilotica which is native to Egypt, with a long history in its tradition, found in its papyri and in folk medicine to treat various ailments. Methods LC–LTQ–MS–MS analysis and 1 H-NMR were used to identify the main phytoconstituents in the n- butanol fraction of T.nilotica fowers. The extract was tested in vitro for its cytotoxic efect against breast (MCF-7) and liver cell carcinoma (Huh-7) using SRB assay. Results T.nilotica n-butanol fraction of the fowers was found to be rich in phenolic content, where, LC–LTQ–MS– MS allowed the tentative identifcation of thirty-nine metabolites, based on the exact mass, the observed spectra fragmentation patterns, and the literature data, varying between tannins, phenolic acids, and favonoids. 1 H-NMR confrmed the classes tentatively identifed. The in-vitro evaluation of the n-butanol fraction showed lower activ‑ ity on MCF-7 cell lines with IC50>100 µg/mL, while the higher promising efect was against Huh-7 cell lines with an IC50= 37 µg/mL. Conclusion Our study suggested that T.nilotica fowers’n-butanol fraction is representing a promising cytotoxic can‑ didate against liver cell carcinoma having potential phytoconstituents with variable targets and signaling pathways.
  • Thumbnail Image
    Item
    The metabolomic analysis of five Mentha species: cytotoxicity, anti-Helicobacter assessment, and the development of polymeric micelles for enhancing the anti-Helicobacter activity
    (Royal Society of chemistry, 1/13/2021) Bakr, Riham O; Tawfike, Ahmed; El-Gizawy, Heba A; Tawfik, Nashwa; Abdelmohsen, Usama Ramadan; Abdelwahab, Miada F.; Alshareef, Walaa A; Fayez, Sahar M.; El-Mancy, Shereen M. S; El-Fishawy, Ahlam M; Abdelkawy, Mostafa A; Fayed, Marwa A. A
    Mentha species are medicinally used worldwide and remain attractive for research due to the diversity of their phytoconstituents and large therapeutic indices for various ailments. This study used the metabolomics examination of five Mentha species (M. suaveolens, M. sylvestris, M. piperita, M. longifolia, and M. viridis) to justify their cytotoxicity and their anti-Helicobacter effects. The activities of species were correlated with their phytochemical profiles by orthogonal partial least square discriminant analysis (OPLS-DA). Tentatively characterized phytoconstituents using liquid chromatography high-resolution electrospray ionization mass spectrometry (LC-HR-ESI-MS) included 49 compounds: 14 flavonoids, 10 caffeic acid esters, 7 phenolic acids, and other constituents. M. piperita showed the highest cytotoxicity to HepG2 (human hepatoma), MCF-7 (human breast adenocarcinoma), and CACO2 (human colon adenocarcinoma) cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. OPLS-DA and dereplication studies predicted that the cytotoxic activity was related to benzyl glucopyranoside-sulfate, a lignin glycoside. Furthermore, M. viridis was effective in suppressing the growth of Helicobacter pylori at a concentration of 50 mg mL1. OPLS-DA predicted that this activity was related to a dihydroxytrimethoxyflavone. M. viridis extract was formulated with Pluronic® F127 to develop polymeric micelles as a nanocarrier that enhanced the anti-Helicobacter activity of the extract and provided minimum inhibitory concentrations and minimum bactericidal concentrations of 6.5 and 50 mg mL1, respectively. This activity was also correlated to tentatively identified constituents, including rosmarinic acid, catechins, carvone, and piperitone oxide.
  • Thumbnail Image
    Item
    Sterols from Centaurea pumilio L. with cell proliferative activity: In vitro and in silico studies
    (2023-04) Fayed, Marwa A. A; Al-Wahaibi, Lamya H; Bakr, Riham O; Nour, Mai S; Basudan, Omer A; Parvez, Mohammad K; Al-Dosari, Mohammed S; Abdel-Mageed, Wael M
    Numerous studies highlighted the impact of natural products, particularly phytosterols, in wound healing while providing less expensive alternatives to che- mically synthesized drugs, with less side effects. Centaurea pumilio L. (family Asteraceae) is a rare and endangered species of genus Centaurea with few reports concerning its chemistry. Our phytochemical investigation for the non-polar fraction of its aerial parts led to the isolation and identification of the new compound (6) identified as stigmast-1,5-dien-3-O-β-D-glucopyranoside along with five known sterols and triterpenes (1–5) identified as taraxas- terol, β-sitosterol, stigmasterol, β-sitosterol glucoside, and stigmasterol-3-O-β-D-glucopyranoside. Structures of the isolated compounds have been characterized using 1D, 2D NMR, and mass spectral analyses. The cell viability and proliferative activity of the isolated compounds were evaluated using an MTT assay on cultured human primary umbilical vein endothelial cells (HUVEC). None of the com- pounds exhibited any sign of cytotoxicity. Nonetheless, com- pounds 5 and 6 moderately enhanced the HUVEC cell growth by 14 and 16%, respectively, at the maximal tested dose (50 µg/mL). As inhibition of glycogen synthase kinase 3-β (GSK3-β) enzyme is important to enhance the wound healing process; therefore, molecular docking was per- formed to understand the possible interactions between bioactive compounds 5 and 6 and GSK-3β binding pocket active amino acid residues. Both compounds were able to bind to the substrate‑binding site of GSK-3β and poten- tially interact with the key active site residues, forming strong π and hydrogen interactions with the catalytic site residues, revealing lower binding energy (−7.185 and −6.303 kcal/mol, respectively) than that of indirubin-3- monooxime (−5.303 kcal/mol); thereby representing strong natural replacements candidates for GSK-3β inhibitors