Browsing by Author "El-Fishawy, Ahlam M"

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    Delineating a potent antiviral activity of Cuphea ignea extract loaded nano-formulation against SARS-CoV-2: In silico and in vitro studies
    (Elsevier, 9/15/2021) Mahmoud, Dina B; Ismail, Walaa M; Moatasim, Yassmin; Kutkat, Omnia; ElMeshad, Aliaa N; Ezzat, Shahira M; El Deeb, Kadriya S; El-Fishawy, Ahlam M; Gomaa, Mokhtar R; Kandeil, Ahmed; Al-karmalawy, Ahmed A; Ali, Mohamed A; Mostafa, Ahmed
    Journal of Drug Delivery Science and Technology Available online 15 September 2021, 102845 In Press, Journal Pre-proofWhat are Journal Pre-proof articles? Delineating a potent antiviral activity of Cuphea ignea extract loaded nano-formulation against SARS-CoV-2: In silico and in vitro studies Author links open overlay panelDina B.MahmoudaWalaa M.IsmailbYassminMoatasimcOmniaKutkatcAliaa N.ElMeshaddeShahira M.EzzatbfKadriya S.El DeebbAhlam M.El-FishawybMokhtar R.GomaacAhmedKandeilcAhmed A.Al-karmalawygMohamed A.AlicAhmedMostafac a Pharmaceutics Department, National Organization for Drug Control and Research, Giza, Egypt b Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo, 11562, Egypt c Center of Scientific Excellence for Influenza Viruses, National Research Centre, Giza, Egypt d Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo, 11562, Egypt e Faculty of Nanotechnology for Postgraduate Studies, Cairo University, El Sheikh Zayed, Giza, 12588, Egypt f Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, 12451, Egypt g Department of Pharmaceutical Medicinal Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta, 34518, Egypt Received 7 March 2021, Revised 17 July 2021, Accepted 5 September 2021, Available online 15 September 2021. https://doi.org/10.1016/j.jddst.2021.102845 Get rights and content Abstract The outbreak of coronavirus disease-2019, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a worldwide emerging crisis. Polyphenols are a class of herbal metabolites with a broad-spectrum antiviral activity. However, most polyphenols encounter limited efficacy due to their poor solubility and degradation in neutral and basic environments. Thus, the effectiveness of their pharmaceutical application is critically dependent on the delivery systems to overcome the aforementioned drawbacks. Herein, Polyphenols-rich Cuphea ignea extract was prepared and its constituents were identified and quantified. Molecular docking was conducted for 15 compounds in the extract against SARS-CoV-2 main protease, among which rutin, myricetin-3-O-rhamnoside and rosmarinic acid depicted the most promising antiviral activity. Further, a self-nanoemulsifying formulation, composed of 10% oleic acid, 40% tween 20 and propylene glycol 50%, were prepared to improve the solubility of the extract components and enable its concurrent delivery permitting combined potency. Upon dilution with aqueous phases, the formulation rapidly form nanoemulsion of good stability and excellent dissolution profile in acidic pH when compared to the crude extract. It inhibited SARS-CoV-2 completely in vitro at a concentration as low as 5.87 μg/mL presenting a promising antiviral remedy for SARS-CoV-2, which may be attributed to the possible synergism between the extract components.
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    The metabolomic analysis of five Mentha species: cytotoxicity, anti-Helicobacter assessment, and the development of polymeric micelles for enhancing the anti-Helicobacter activity
    (Royal Society of chemistry, 1/13/2021) Bakr, Riham O; Tawfike, Ahmed; El-Gizawy, Heba A; Tawfik, Nashwa; Abdelmohsen, Usama Ramadan; Abdelwahab, Miada F.; Alshareef, Walaa A; Fayez, Sahar M.; El-Mancy, Shereen M. S; El-Fishawy, Ahlam M; Abdelkawy, Mostafa A; Fayed, Marwa A. A
    Mentha species are medicinally used worldwide and remain attractive for research due to the diversity of their phytoconstituents and large therapeutic indices for various ailments. This study used the metabolomics examination of five Mentha species (M. suaveolens, M. sylvestris, M. piperita, M. longifolia, and M. viridis) to justify their cytotoxicity and their anti-Helicobacter effects. The activities of species were correlated with their phytochemical profiles by orthogonal partial least square discriminant analysis (OPLS-DA). Tentatively characterized phytoconstituents using liquid chromatography high-resolution electrospray ionization mass spectrometry (LC-HR-ESI-MS) included 49 compounds: 14 flavonoids, 10 caffeic acid esters, 7 phenolic acids, and other constituents. M. piperita showed the highest cytotoxicity to HepG2 (human hepatoma), MCF-7 (human breast adenocarcinoma), and CACO2 (human colon adenocarcinoma) cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. OPLS-DA and dereplication studies predicted that the cytotoxic activity was related to benzyl glucopyranoside-sulfate, a lignin glycoside. Furthermore, M. viridis was effective in suppressing the growth of Helicobacter pylori at a concentration of 50 mg mL1. OPLS-DA predicted that this activity was related to a dihydroxytrimethoxyflavone. M. viridis extract was formulated with Pluronic® F127 to develop polymeric micelles as a nanocarrier that enhanced the anti-Helicobacter activity of the extract and provided minimum inhibitory concentrations and minimum bactericidal concentrations of 6.5 and 50 mg mL1, respectively. This activity was also correlated to tentatively identified constituents, including rosmarinic acid, catechins, carvone, and piperitone oxide.