Browsing by Author "El-Borady, Ola M"

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Evaluation of the Potential Role of Silver Nanoparticles Loaded with Berberine in Improving Anti-Tumor Efficiency
(Tumos. Press, 2022-03) Othman, Mohamed S; Obeidat, Sofian T; Al-Bagawi, Amal H; Fareid, Mohamed A; El-Borady, Ola M; Kassab, Rami B; Abdel Moneim, Ahmed E
Background: Cancer is a progressive disease, its incidence and death rates are rapidly increasing globally. Numerous adverse effects are associated with the available interventions. Hence, the current study was undertaken to explore the anticancer effect of silver nanoparticles conjugated with berberine (AgNPs-BER) against Ehrlich solid carcinoma (ESC) in mice. Methods: Male Swiss albino mice were allocated randomly into ESC, ESC+cisplatin (CP; 5 mg/ kg), ESC+AgNPs-BER (20 mg/kg), and ESC+cisplatin and AgNPs-BER groups. Results: AgNPs-BER administration increased significantly the survival rate and decreased body weight and tumor size as compared to ESC group. Additionally, AgNPs-BER enhanced the development of oxidative stress in the tumor tissue as indicated by the increased lipid peroxidation (LPO) and nitric oxide (NO) accompanied by a decrease in the examined antioxidant proteins (glutathione (GSH) and its derived enzymes along with superoxide dismutase and catalase). AgNPs-BER was found also to trigger apoptotic cascade in the tumor cells through upregulating the mRNA expression of the pro-apoptotic proteins (Bax and caspase-3) and downregulating the mRNA expression of the anti-apoptotic protein (Bcl-2). Moreover, AgNPs-BER improved partially the histopathological alterations in the developed tumor tissue as compared to ESC group. Conclusion: Collectively, AgNPs-BER could be applied as an antitumor agent due to its pro- oxidant, pro-apoptotic, and antiangiogenic effects.
Physical and mechanical evaluation of dental resin composite after modification with two different types of Montmorillonite nanoclay
(Elsevier, 6/27/2021) Elmergawy, Fagr Hassan; Nassif, Mohammed Salah; El-Borady, Ola M; Mabrouk, Mostafa
Objectives: Evaluation of degree of conversion (DC), flexural properties, micro-hardness and color change (ΔE00) of dental resin composite after modification with two types of organo-modified Montmorillonite (MMT) nano- clay; an experimentally synthesized polymethyl-methacrylate modified MMT nanoclay (PMMA/MMT), and a commercially available one (Cloisite20A). Methods: MMT was synthesized by sol-gel technique, organo-modified with polymethyl-methacrylate and char- acterized using EDX, XRD and FTIR. PMMA/MMT and Cloisite20A nanoclay were added to flowable resin composite in 0.5, 1 and 1.5 wt% concentrations. Unmodified resin composite was used as control group. DC was assessed by FTIR, flexural properties were tested by three-point bending test using a universal testing machine, micro-hardness was analyzed by Vickers micro-hardness tester and color change (ΔE00) was evaluated using a reflective spectrophotometer. SEM and elemental mapping assessment were performed to evaluate nanoclay distribution in resin composite. Data were analyzed using One-way ANOVA followed by Tukey’s post hoc test, in addition to Two-way ANOVA (p ≤ 0.05). Weibull analysis was used to analyze flexural strength results. Results: Characterization results revealed successful preparation of PMMA/MMT. DC results showed insignificant difference up to 1 wt% of nanoclay concentration. Addition of 0.5 wt% of PMMA/MMT significantly increased flexural properties, while addition of 1.5 wt% of PMMA/MMT significantly decreased flexural properties. Micro- hardness results revealed a significant increase in PMMA/MMT groups in all tested concentrations. ΔE00 results showed that color change was clinically acceptable on adding 0.5 wt% nanoclay. Conclusion: PMMA/MMT in 0.5 wt% is a promising nanofiller for resin composite that significantly enhanced flexural strength and micro-hardness without compromising DC and color.
REMAKABLE ENHANCEMENT OF CYHALOTHRIN UPON LOADING INTO SILVER NANOPARTICLES AS LARVICIDAL
(IJCAS, 2016-01) Abouelkassem, Sherif; El-Borady, Ola M; Mohamed, Mona B.
This work has been devoted to develop a novel synthetic scheme to produce pesticide nanocomposite of very high efficiency compared to its original ones. laboratory experiments conducted in NanoTech. company, Dreamland, October city, Egypt and Department of plant protection, Fac. of Agric. Alazhar Univ. Assiut. Egypt. The method is based on using silver nanoparticles (AgNPS) as a pesticide carrier by loading pyrethroid pesticide cyhalothrin (CYN) into the surface of prepared AgNPS. The nature of binding of the pesticide into the nanoparticles surface has been investigated via TEM and FT-IR techniques. The new formulation of the pesticide nanocomposite AgNPS@CYN has been tested for its larvicidal property against three strains larvae of Culex pipiens (C. pipiens) (i.e. susceptible, field and tolerant strains). Our findings indicate that silver- cyhalothrin nanocomposite is more efficient in controlling mosquito larvae than that of free cyhalothrin. The required concentration for mosquito control decrease more than 100 times. This approach might be successful ones for safer pesticide and clean for the environment.
Response surface optimised photocatalytic degradation and quantitation of repurposed COVID-19 antibiotic pollutants in wastewaters; towards greenness and whiteness perspectives
(CSIRO, 2023-12) Elbalkiny, Heba T; El-Borady, Ola M; Saleh, Sarah S; El-Maraghy, Christine M
Rationale. Certain antibiotics have been repurposed for the management of infected COVID-19 cases, because of their possible effect against the virus, and treatment of co-existing bacterial infection. The consumption of these antibiotics leads to their access to sewage, industrial and hospital effluents, then to environmental waters. This creates a need for the routine analysis and treatment of water resources. Methodology. Detection and quantitation of three repurposed antibiotics: levofloxacin (LEVO), azithromycin (AZI) and ceftriaxone (CEF) were studied in different water samples using LC-MS/MS methods employing a C18 column and a mobile phase consisting of 80% acetonitrile/20% (0.1% formic acid in water) after solid phase extraction on Oasis HLB Prime cartridges. Real water samples were treated with synthesised graphitic carbon nitride (g-C3N4) to remove the three types of antibiotics from contaminated water under experimental conditions optimised by response surface methodology, using Box–Behnken experimental design. Results. The analytical method was validated in the concentration range of 10–5000 ng mL–1 for the three drugs. The removal percentages were found to be 92.55, 98.48 and 99.10% for LEVO, AZI and CEF, respectively, using synthesised g-C3N4. Discussion. The analytical method was used for the estimation of the three cited drugs before and after their removal. The method was assessed using ComplexGAPI as a greenness tool and the RGB 12 algorithm as a whiteness model. The method was applied for the analysis and treatment of real water samples before and after their treatment. It proved to be simple, low-cost and environmentally sustainable.