Browsing by Author "Dawoud, Marwa H. S"

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    A Quality by Design Paradigm for Albumin‑Based Nanoparticles: Formulation Optimization and Enhancement of the Antitumor Activity
    (Springer New York, 2023-05) Dawoud, Marwa H. S; Abdel‑Daim, Amira ; Nour, Mai S; Sweed, Nabila M
    Albumin nanoparticles are promising carriers for therapeutic agents, owing to their biocompatibility, safety, and versatility in fabrication. The formulation of albumin nanoparticles is highly afected by many product and process variables, resulting in a great variation in these nanoparticles. The aim of this work was to formulate and optimize albumin nanopar- ticles loaded with silymarin, as a model drug with low bioavailability, for the treatment of hepatocellular carcinoma, using quality by design (QbD) approach. Methods A thorough risk assessment for albumin nanoparticles formulation was developed and a complete quality product profle was defned using the QbD approach. A D-optimal design was used to optimize the amount of albumin and drug, which signifcantly afected the particle size (PS) and the entrapment efciency (EE%), which was further tested on hepa- tocellular carcinoma. Results A design space was constructed, with an optimized formula showing a PS of 135 nm, a polydispersity index (PDI) of 0.09, an EE% of 88%, and a zeta potential of−12.5 mV. The optimized formula (O1) with spherical particles, showed an extended-release rate as compared to free silymarin. Moreover, a pronounced anti-proliferation activity of O1 was observed on human hepatocellular carcinoma cell line HepG2 than the free drug. The fow cytometric analysis of the cell cycle showed a signifcant suppression of the S-phase after treating the HepG2 cell with O1, but not with free silymarin. Conclusion Thus, a detailed QbD study has been conducted, with deep product and process understanding, and resulted in a successful formulation of silymarin albumin nanoparticles for the suppression of hepatocellular carcinoma.
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    Response surface optimization of a cardioprotective compound through pharmacosomal drug delivery system: in vivo bioavailability and cardioprotective activity potential
    (Springer Publishing Company, 2023-04) Dawoud, Marwa H. S; Zaafan, Mai A; Saleh, Sarah S; Mannaa, Islam M; Sweed, Nabila M
    Vanillic acid (VA) is a phenolic compound with potential antioxidant activity, which improves ischemia-induced myocardial degeneration, by reducing oxidative stress; however, it sufers poor bioavailability owing to its poor solubility. VA-loaded pharmacosomes were optimized using a central composite design, where the efect of phosphatidylcholine:VA molar ratio and the precursor concentration were studied. An optimized formulation (O1) was prepared and tested for the release rate of VA, in vivo bioavailability, and cardioprotective potential on myocardial infarction-induced rats. The optimized formulation showed a particle size of 229.7 nm, polydispersity index of 0.29, and zeta potential of−30 mV. O1 showed a sustained drug release for 48 h. The HPLC–UV method was developed for the determination of VA in plasma samples using protein pre- cipitation. The optimized formulation showed a great improvement in the bioavailability as compared to VA. The residence time of the optimized formula was 3 times longer than VA. The optimized formulation showed a more potent cardioprotec- tive efect as compared to VA, via inhibition of the MAPK pathway with subsequent inhibition of PI3k/NF-κB signaling, in addition to its antioxidant efect. The optimized formulation showed normalization of many oxidative stress and infamma- tory biomarkers. Thus, a VA-loaded pharmacosome formulation with promising bioavailability and cardioprotective activity potential was prepared.