Browsing by Author "Ashour M.S.E.-D."

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Antimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa strains isolated from cancer patients
(2014) Zafer M.M.; Al-Agamy M.H.; El-Mahallawy H.A.; Amin M.A.; Ashour M.S.E.-D.; Department of Microbiology and Immunology; Faculty of Pharmacy; Ahram Canadian University; Cairo; Egypt; Department of Pharmaceutics and Microbiology; College of Pharmacy; King Saud University; P.O. Box 2457; Riyadh 11451; Saudi Arabia; Department of Microbiology and Immunology; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Clinical Pathology; National Cancer Institute; Cairo University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Science and Arts University; 6th October City; Giza; Egypt
This study was designed to investigate the prevalence of metallo-?-lactamases (MBL) and extended-spectrum ?-lactamases (ESBL) in P. aeruginosa isolates collected from two different hospitals in Cairo, Egypt. Antibiotic susceptibility testing and phenotypic screening for ESBLs and MBLs were performed on 122 P. aeruginosa isolates collected in the period from January 2011 to March 2012. MICs were determined. ESBLs and MBLs genes were sought by PCR. The resistant rate to imipenem was 39.34%. The resistance rates for P. aeruginosa to cefuroxime, cefoperazone, ceftazidime, aztreonam, and piperacillin/tazobactam were 87.7%, 80.3%, 60.6%, 45.1%, and 25.4%, respectively. Out of 122 P. aeruginosa, 27% and 7.4% were MBL and ESBL, respectively. The prevalence of bla VIM2, bla OXA10-, bla VEB1, bla NDM-, and bla IMP1-like genes were found in 58.3%, 41.7%, 10.4%, 4.2%, and 2.1%, respectively. GIM-, SPM-, SIM-, and OXA-2-like genes were not detected in this study. OXA-10-like gene was concomitant with VIM-2 and/or VEB. Twelve isolates harbored both OXA-10 and VIM-2; two isolates carried both OXA-10 and VEB. Only one strain contained OXA-10, VIM-2, and VEB. In conclusion, bla VIM2- and bla OXA10-like genes were the most prevalent genes in P. aeruginosa in Egypt. To our knowledge, this is the first report of bla VIM2, bla IMP1, bla NDM, and bla OXA10 in P. aeruginosa in Egypt. � 2014 Mai M. Zafer et al.
Microbial evaluation of some non-sterile pharmaceutical preparations commonly used in the Egyptian market
(University of Benin, 2011) Gad G.F.M.; Aly R.A.I.; Ashour M.S.E.-D.; Microbiology Department; Faculty of Pharmacy; Minia University; Minia; Egypt; Microbiology Department; Faculty of Pharmacy; Modern Science and Arts University; Cairo; Egypt
Purpose: To determine the type and incidence of predominant microorganisms in certain non-sterile pharmaceuticals immediately after collection and one year later. Methods: All pharmaceutical samples were subjected to the following examinations: total bacterial count and presence of microbial pathogens, using conventional techniques. Attempts were also made to identify the isolates. The bioburden rate of some of the syrups and oral drops after storage for 0, 6 and 12 months were evaluated in order to assess the effect of storage on microbial contamination level. Results: Microbial load varied among the pharmaceutical preparations with the highest microbial load in suspensions and the lowest in tablets. Bacterial counts ranged from 10 to more than 103 CFU per ml or g. The bacterial count at 6 and 12 months were significantly different from that at 0 month (p < 0.05). The isolated organisms were either of human flora types, essentially Gram-positive bacteria, or airborne fungi. Conclusion: The isolated organisms were either of human flora types, principally, Gram-positive bacteria, or air-borne fungi and the stored preparations lack an effective preservation. Several measures, including equipment automation, monitoring programs and post-marketing surveillanceare required to reduce the level of microbial contamination of non-sterile pharmaceutical products. � Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City.