Browsing by Author "Amin M.A."

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Antimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa strains isolated from cancer patients
(2014) Zafer M.M.; Al-Agamy M.H.; El-Mahallawy H.A.; Amin M.A.; Ashour M.S.E.-D.; Department of Microbiology and Immunology; Faculty of Pharmacy; Ahram Canadian University; Cairo; Egypt; Department of Pharmaceutics and Microbiology; College of Pharmacy; King Saud University; P.O. Box 2457; Riyadh 11451; Saudi Arabia; Department of Microbiology and Immunology; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Clinical Pathology; National Cancer Institute; Cairo University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Science and Arts University; 6th October City; Giza; Egypt
This study was designed to investigate the prevalence of metallo-?-lactamases (MBL) and extended-spectrum ?-lactamases (ESBL) in P. aeruginosa isolates collected from two different hospitals in Cairo, Egypt. Antibiotic susceptibility testing and phenotypic screening for ESBLs and MBLs were performed on 122 P. aeruginosa isolates collected in the period from January 2011 to March 2012. MICs were determined. ESBLs and MBLs genes were sought by PCR. The resistant rate to imipenem was 39.34%. The resistance rates for P. aeruginosa to cefuroxime, cefoperazone, ceftazidime, aztreonam, and piperacillin/tazobactam were 87.7%, 80.3%, 60.6%, 45.1%, and 25.4%, respectively. Out of 122 P. aeruginosa, 27% and 7.4% were MBL and ESBL, respectively. The prevalence of bla VIM2, bla OXA10-, bla VEB1, bla NDM-, and bla IMP1-like genes were found in 58.3%, 41.7%, 10.4%, 4.2%, and 2.1%, respectively. GIM-, SPM-, SIM-, and OXA-2-like genes were not detected in this study. OXA-10-like gene was concomitant with VIM-2 and/or VEB. Twelve isolates harbored both OXA-10 and VIM-2; two isolates carried both OXA-10 and VEB. Only one strain contained OXA-10, VIM-2, and VEB. In conclusion, bla VIM2- and bla OXA10-like genes were the most prevalent genes in P. aeruginosa in Egypt. To our knowledge, this is the first report of bla VIM2, bla IMP1, bla NDM, and bla OXA10 in P. aeruginosa in Egypt. � 2014 Mai M. Zafer et al.
Molecular characterization of Enterococcus spp. clinical isolates from Cairo, Egypt
(Medknow Publications, 2015) Hashem Y.A.; Yassin A.S.; Amin M.A.; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Sciences and Arts University; Giza; Egypt; Cairo University; Cairo; Egypt
Purpose: Enterococci are responsible for serious diseases such as bacteraemia, endocarditis and urinary tract infections. The ability of enterococci to cause such diseases is due to acquisition of certain virulence factors such as haemolysin, gelatinase and enterococcus surface protein. This study has been conducted to investigate the occurrence of virulence factors and resistance to various antibiotics with emphasis on vancomycin in the Enterococcus spp. Materials and Methods: Clinical specimens were collected and isolates were identified by proper microscopic, culture and biochemical tests. Susceptibility and degree of resistance of the isolates to various antibiotics were determined. Virulence factors were examined by phenotypic tests followed by molecular methods. Bioinformatics analysis was used to detect regions in the genomes that might have originated from horizontal gene transfer. Result: The presence or absence of virulence genes did not affect the pattern of antimicrobial resistance in Enterococcus isolates; consequently, no relationship was found between virulence factors and resistance to different antibiotics used. Bioinformatics analysis showed that the virulence genes were mainly transferred by transposons. Conclusion: Among the enterococci, environmental factors may interfere in the expression of virulence factors. Horizontal gene transfer plays an important role in the spread of resistance and virulence genes.