Browsing by Author "Amer, Reham I"

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Chewing gum containing repaglinide solid dispersion using mesoporous silica nanoparticles for management of diabetes mellitus: In-vitro evaluation and clinical appraisal
(Editions de Sante, 2024-02) Badawi, Noha M; Amer, Reham I; Attia, Dalia A; Fayez, Ahmed M; Dawoud, Marwa H.S
The current study aimed to formulate repaglinide solid dispersion via mesoporous silica nanoparticles (R-MSNs) as a carrier in an attempt to enhance drug solubility. Then incorporation of it in a medicated chewing gum (MCG); to enhance the drug bioavailability, for the treatment of diabetes mellitus. R-MSNs solid dispersion formulations (R-MSNs-SD) were prepared using the solvent evaporation method. The chosen formula was investigated in terms of FTIR, DSC, and XRD in addition to morphology that was studied by SEM. The results demonstrated that repaglinide was successfully loaded into the pores of MSNs. The cytotoxicity of the chosen formula was evaluated by SRB assay using a Vero cell line and the cytotoxic effect of repaglinide was found to be diminished when incorporated within MSNs. Subsequently, MCGs were formulated using the chosen R-MSNs-SD as well as pure repaglinide and tested for physical properties, content uniformity, and drug release. The results exhibited a notable improvement in the release of repaglinide from the MCG containing pure repaglinide and RMSNs-SD. A clinical investigation was further conducted on diabetic patients for MCGs containing R-MSNs-SD or pure repaglinide and compared to the marketed product where the blood glucose level was measured. MCG formulations specially the one loaded with R-MSNs-SD showed enhanced antidiabetic activity than the marketed product suggesting a promising oral antidiabetic delivery system for repaglinide.
Downregulation of MMP1 expression mediates the anti-aging activity of Citrus sinensis peel extract nanoformulation in UV induced photoaging
(Elsevier, 2021-03) Amer, Reham I; Ezzat, Shahira M; Aborehab, Nora M; Ragab, Mai F; Mohamed, Dalia; Hashad, Amira; Attia, Dalia; Salama, Maha M; El Bishbishy, Mahitab H
Aging of the skin is a complicated bioprocess that is affected by constant exposure to ultraviolet irradiation. The application of herbal-based anti-aging creams is still the best choice for treatment. In the present study, Citrus sinensis L. fruit peels ethanolic extract (CSPE) was formulated into lipid nanoparticles (LNPs) anti-aging cream. Eight different formulations of CSEP-LNPs were prepared and optimized using 23 full factorial designs. In vivo antiaging effect of the best formula was tested in Swiss albino mice where photo-aging was induced by exposure to UV radiation. HPLC-QToF-MS/MS metabolic profiling of CSPE led to the identification of twenty-nine me- tabolites. CSPE was standardized to a hesperidin content of 15.53 ± 0.152 mg% using RP-HPLC. It was suggested that the optimized formulation (F7) had (245 nm) particle size, (91.065%) EE, and (91.385%) occlusive effect with a spherical and smooth surface. The visible appearance of UV-induced photoaging in mice was significantly improved after topical application on CSPE-NLC cream for 5 weeks, levels of collagen and SOD were significantly increased in CSPE- NLC group, while levels of PGE2, COX2, JNK, MDA, and elastin was reduced. Finally, The prepared anti-aging CSPE-NLC cream represents a safe, convenient, and promising skincare cosmetic product.
Formulation, characterization and cellular toxicity assessment of a novel bee‑venom microsphere in prostate cancer treatment
(Springer, 2022-07) El‑Didamony, Samia E; Amer, Reham I; El‑Osaily, Ghada H
Bee venom (B.V.) is a toxin produced naturally by honey bees with several toxic and therapeutic efcacies. It is used in the treatment of diferent cancer kinds like renal, hepatic, and prostate cancer. Due to its protein nature, it is degraded in the upper gastrointestinal tract. Colon-targeted drug delivery systems represent a useful tool to protect B.V. from degradation and can be administered orally instead of I.V. infusion and traditional bee stinging. In the present study, B.V. loaded enteric- coated cross-linked microspheres were prepared by emulsion cross-linking method. Percentage yield, entrapment efciency %, swelling degree, and in-vitro release are evaluated for prepared microspheres. Free B.V., optimized microspheres formula (F3), and doxorubicin cytotoxic efects were tested by MTT assay. Results concluded that free B.V. was more efective against the growth of human prostate adenocarcinoma (PC3) cells followed by optimized microspheres than doxorubicin. But both free B.V. and doxorubicin have a cytotoxic efect on normal oral epithelial cells (OEC). According to fow cytometric analysis, the optimized microsphere formula induced apoptosis and reduced necrosis percent at IC50 concentration. Furthermore, microspheres did not afect the viability of OEC. These results revealed that microspheres have a degree of specifcity for malignant cells. Therefore, it seems that this targeted formulation could be a good candidate for future clinical trials for cancer therapy.
Pharmaceutical and Pharmacological Evaluation of the Effect of Nano-Formulated Spironolactone and Progesterone on Inflammation and Hormonal Levels for Managing Hirsutism Experimentally Induced in Rats
(Springer, 7/13/2021) Amer, Reham I; Yassin, Ghada E; Mohamed, Reem A; Fayez, Ahmed M
Hirsutism is a dermatological condition that refers to the excessive growth of hair in androgen-sensitive areas in women. Recently, the enhancement of the visible signs of a hairy female has taken special concern that affected the quality of life. The present study was developed to compare the follicular targeting effect of topical spironolactone (SP) or progesterone (PG)-loaded nanostructured lipid carrier (NLC) on the management of hirsutism. Four NLC formulations were prepared using cold homogenization techniques and pharmaceutically evaluated. SP-NLC and PG-NLC topical hydrogels were prepared to explore their pharmacological effect on letrozole induced polycystic ovarian syndrome (PCOS) in rats. Inflammatory mediators, antioxidant, and hormonal parameters were assayed. Additionally, histopathological examination was carried out to confirm the successful induction of PCOS. Results confirmed that all NLC formulations have a spherical shape with particle size ranged from 225.92 ± 0.41 to 447.80 ± 0.66 nm, entrapment efficiency > 75%, and zeta potential (− 31.4 to − 36.5 mV). F1 and F3 NLCs were considered as selected formulations for SP and PG, respectively. Female Wistar rats treated with F1 formulation for 3 weeks displayed better outcomes as manifested by the measured parameters as compared to the other tested groups. A significant reduction in hair follicle diameter and density was observed after topical application of SP or PG nano-gels. Finally, the outcomes pose a strong argument that the development of topically administered SP- NLC can be explored as a promising carrier over PG-NLC for more effectual improvement in the visible sign of hirsutism.