Browsing by Author "Aboulwafa M.M."

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Antagonistic activity of lactobacillus isolates against salmonella typhi in vitro
(2013) Abdel-Daim A.; Hassouna N.; Hafez M.; Ashor M.S.A.; Aboulwafa M.M.; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo 12611; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Ain Shams University; Al Khalifa Al Maamoun Street; Abbassia; Cairo 11566; Egypt; Department of Pharmaceutical Microbiology; College of Pharmacy; Taif University; Saudi Arabia
Background. Enteric fever is a global health problem, and rapidly developing resistance to various drugs makes the situation more alarming. The potential use of Lactobacillus to control typhoid fever represents a promising approach, as it may exert protective actions through various mechanisms. Methods. In this study, the probiotic potential and antagonistic activities of 32 Lactobacillus isolates against Salmonella typhi were evaluated. The antimicrobial activity of cell free supernatants of Lactobacillus isolates, interference of Lactobacillus isolates with the Salmonella adherence and invasion, cytoprotective effect of Lactobacillus isolates, and possibility of concurrent use of tested Lactobacillus isolates and antibiotics were evaluated by testing their susceptibilities to antimicrobial agents, and their oxygen tolerance was also examined. Results. The results revealed that twelve Lactobacillus isolates could protect against Salmonella typhi infection through interference with both its growth and its virulence properties, such as adherence, invasion, and cytotoxicity. These Lactobacillus isolates exhibited MIC values for ciprofloxacin higher than those of Salmonella typhi and oxygen tolerance and were identified as Lactobacillus plantarum. Conclusion. The tested Lactobacillus plantarum isolates can be introduced as potential novel candidates that have to be subjected for in vivo and application studies for treatment and control of typhoid fever. � 2013 Amira Abdel-Daim et al.
Comparative study on serodiagnostic techniques of Brucella infection in Egypt
(2012) Basyony A.F.; Aboulwafa M.M.; Hafez M.M.; Abou Gazia K.A.; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Sciences and Arts University; Egypt; Microbiology and Immunology Department; Faculty of Pharmacy; Ain Shams University; Egypt; Department of Microbiology and Immunology; Animal Reproduction Research Institute; Agriculture Research Center; Egypt
Background: Brucellosis is a zoonotic disease that affects wild and domestic animals causing a decrease in reproductive efficiency and abortion and can be transmitted to human. The incidence of human disease is closely tied to the prevalence of infection in animals and considered as an important health problem in Egypt. Methods and Findings: In this study, blood specimens from 68 patients that showed clinical signs and/or history of brucellosis and from different investigated animals (76 buffalo, 145 cattle and 191 sheep) were collected and serodiagnosed for Brucella infection. The sera of these blood specimens were first screened by rose bengal plate test (RBPT) and those giving positive reaction were retested by the standard tube agglutination test (SAT), EDTA modified SAT and rivanol test to determine their titers. The results for clinical specimens showed that 89.70%, 82.35%, 66.18% and 58.82% were positive using RBPT, SAT, EDTA modified SAT and rivanol test, respectively. The respective percentages of brucellosis in buffalo were 44.70%, 43.42%, 43.42% and 43.24%; while the respective percentages of brucellosis in cattle were 46.90%, 43.45%, 39.31% and 37.93%. In addition, serological examination of 191 sheep revealed that 60.20%, 56.54%, 53.40% and 51.83% were positive using RBPT, SAT, EDTA modified SAT and rivanol test, respectively. Conclusion: The results give clear evidence for: (i) the real picture of brucellosis surveillance among human cannot be reflected using single serodiagnostic test, (ii) In comparison to human, serodiagnosis of Brucella among animals is less dependent on test type and such dependency took the order sheep > cattle > buffalo, (iii) serodiagnosis of Brucella among buffalo had nearly no dependency on test type. � Under License of Creative Commons Attribution 3.0 License.
Screening of Lactobacillus isolates for their adherence capabilities to mammalian cells and their acid and bile tolerance
(2012) Abdel-Daim A.; Hassouna N.; Hafez M.; Ashor M.S.A.; Aboulwafa M.M.; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Ain Shams University; Cairo; Egypt; Department of Pharmaceutical Microbiology; College of Pharmacy; Taif University; Saudi Arabia
Background: Lactobacilli resemble a major part of the commensal human mucosal flora. The application of Lactobacilli as probiotics has increased during the last few years since a health promoting effect has been reported in addition to their long history of safe use. Methods and finding: In this study, fifty-two Lactobacillus isolates were recovered from dairy products or infant stools were examined in vitro for their probiotic potential. Their adherence capacities to Vero cells in addition to their acid and bile tolerance were evaluated. Only few isolates weren't able to adhere to Vero cells, while the other isolates have high to moderate adherence capacities. The majority of isolates were tolerant to acid and about 70% were tolerant to 0.3% bile salts. Conclusion: thirty two Lactobacillus isolates were found to possess desirable probiotic properties. These isolates are good candidates for further investigation in in vitro and in vivo studies for their potential health benefits and their application as novel Biotherapeutic agents. � iMedPub.