Browsing by Author "Abdelhamid, Amr M"

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Association of Apelin Genetic Variants with Type Two Diabetes Mellitus in Egyptian Population.
(A SciTechnol Journal, 2019) Abdelhamid, Amr M; Hafez, Mohamed M; Ibrahim, Sherine M
Background and Aim: Apelin, the newly identified adipokine and the endogenous ligand for the APJ receptor is related to obesity and insulin resistance. The aim of the study was to investigate the association of 2 single-nucleotide polymorphisms (SNPs) in the apelin gene (APLN) with susceptibility to Type Two Diabetes Mellitus (T2DM) in the Egyptian population. Methods: Two SNPs on APLN were genotyped in 145 diabetic patients and 135 nondiabetic individuals, aged 40-60 years. Realtime polymerase chain reaction (RT-PCR) was used to analyze the 2 SNPs in both the diabetic and the healthy subjects. The association of the 2 SNPs (rs2281068 and rs3115759) in APLN and T2DM risk was investigated. Allele and genotype frequencies between patients and control groups were compared using the Chisquare (χ2 ) test. Results: In the apelin gene; GT/TT genotype of apelin risk genotypes of the rs2281068 variants was found to be significantly related with the risk of T2DM with the power (OR : 9.623, CI : 35.52 - 16.77) (P ≤ 0.001). while on the contrary, the GA/AA genotype of the rs3115759 variants was not at increased risk for T2DM (OR :1.25, CI : 0.785-2.09) (P=0.3408). Conclusions: Both association and functional studies suggested that SNP rs2281068 in APLN is associated with the risk of T2DM in the Egyptian population.
Association of Chemerin rs17173608 and Vaspin rs2236242 Polymorphisms with Type Two Diabetes Mellitus and its Impact on their Corresponding Serum Levels in Egyptian Population
(ideas, 2019) Abdelhamid, Amr M; Zaafan, Mai A.
Type 2 diabetes mellitus (T2DM) is a metabolic pro-inflammatory disorder characterized by chronic hyperglycemia and alteration in circulating adipokines.
Dasatinib ameliorates thioacetamide-induced liver fibrosis: modulation of miR-378 and miR-17 and their linked Wnt/b-catenin and TGF-b/smads pathways
(Taylor and Francis, 11/12/2021) Zaafan, Mai A; Abdelhamid, Amr M
Hepatic stellate cells activation (HSCs) plays a crucial role in the pathogenesis of liver fibrosis. Specific microRNAs have been suggested to affect the activation of HSCs via various signalling pathways including TGF-b/smads and Wnt/b-catenin pathways. Dasatinib is a multitarget inhibitor of many tyrosine kinases has recently studied for its anti-fibrotic effects in a variety of fibrous diseases. This study investigated the role of modulation of miRNA-378 and miRNA-17 in the pathogenesis of liver fibrosis through altering Wnt/b-catenin and TGF-b/smads pathways and evaluated the beneficial effect of the tyrosine kinase inhibi- tor, dasatinib, in thioacetamide-induced liver fibrosis model in mice. Treatment with dasatinib down-regu- lated miRNA-17 expression, leading to the restoration of WiF-1 and smad-7 which cause the inhibition of both Wnt/b-catenin and TGF-b/smads signalling. In addition, it upregulated miRNA-378 leading to the decrease of Wnt-10 which contributes to the suppression of activated HSCs.
The Hepatoprotective Effect of Piperine Against Thioacetamide-Induced Liver Fibrosis in Mice: The Involvement of miR-17 and TGF-β/Smads Pathways
(Frontiers Media S.A., 29/10/2021) Abdelhamid, Amr M; Selim, Ayman; Zaafan, Mai A
Liver fibrosis is characterized by a series of events including activation of quiescent hepatic stellate cells (HSCs) into proinflammatory, contractile, and fibrogenic myofibroblasts, which is the primary trigger for the fibrogenesis process. HSC activation involves many signaling pathways such as the TGF-β/smads pathway. Specific microRNAs have been identified to play a crucial role in the activation of HSCs via various signaling pathways. Piperine has recently been studied as a promising anti-fibrotic agent against pancreatic fibrosis through altering the TGF-β1/Smad pathway. Hence, the current study evaluated the beneficial effects of piperine in thioacetamide-induced liver fibrosis in mice through the modulation of miRNA-17 and TGF-β/smads pathways. Mice were allocated into three groups randomly. Thioacetamide was used to induce liver fibrosis for 6 weeks. Starting from the fourth week of the experiment, mice were treated with piperine daily for 21 days. Piperine treatment resulted in a significant downregulation of miRNA-17 expression, leading to the restoration of smad-7 accompanied with marked inhibition of TGF-β/smads signaling with further suppression of the activated HSCs and collagen deposition in the hepatocytes. In conclusion, piperine has the potential to be a promising therapeutic drug for the treatment of liver fibrosis through inhibiting the TGF-β/smads pathway. Copyright © 2021 Abdelhamid, Selim and Zaafan.
The impact of lncRNA-GAS5/miRNA-200/ACE2 molecular pathway on the severity of COVID-19
(Bentham Science Publishers B.V., 2023-05) Ayeldeen, Ghada; Shaker, Olfat G; Amer, Eman; Zaafan, Mai A; Herzalla, Mohamed R; Keshk, Mofida A; Abdelhamid, Amr M
was reported to be overexpressed and its targeted ACE2 was down-regulated. The ROC curve was drawn to examine the diagnostic abilities of GAS5, miR-200, and ACE2, yielding high diagnostic accuracy with high sensitivity and specificity. Conclusion: lncRNA-GAS5, miRNA-200, and ACE2 panels presented great diagnostic potential as they demonstrated the highest diagnostic accuracy for discriminating moderate COVID-19 and severe COVID-19 cases. :
Implication of miR-122, miR-483, and miR-335 Expression Levels as Potential Signatures in HCV-Related Hepatocellular Carcinoma (HCC) in Egyptian Patients
(Frontiers, 2022-05) Elfert, Ashraf Y; Salem, Amel; Abdelhamid, Amr M; Salama, Ahmad; Sourour, Doaa A; Shaker, Olfat; Keshk, Mofida
Hepatocellular carcinoma (HCC) is the second most common cause of cancer-related deaths worldwide with chronic hepatitis C virus (HCV) infection as a major risk factor of HCC. Circulating microRNAs are deregulated in HCC and are candidate biomarkers. The aim of this study was to explore the expression profile of miRNA-122, miR-483, and miR- 335 in the serum of HCV-related hepatocellular carcinoma (HCC). 90 HCV-related hepatocellular carcinoma (HCC) patients, 90 non-malignant HCV patients, and 60 healthy controls were included. Serum microRNAs were measured by a qRT-PCR custom array. The expression levels of miR-122 and miR-483 were upregulated in HCC patients, while the miR-335 expression level was downregulated versus controls and HCV groups. Receiver-operating characteristic (ROC) curve analysis was created to examine miRNAs. miR-483 presented the best diagnostic potential because it showed the highest diagnostic accuracy for distinguishing HCV-related HCC patients from controls (AUC = 0.98) with 100% sensitivity. Moreover, there was obvious prognostic power in distinguishing HCV from HCC (AUC = 0.95) with 88% sensitivity. In conclusion, studied microRNAs (miR-122, miR-483, and miR-335) could serve as potential non-invasive early diagnostic biomarkers for HCC, and we identified a panel of three serum microRNAs with high accuracy in HCC diagnosis. Additional studies are required to confirm this panel and test its prognostic significance.
Modulation of the miR-122/Sirt-6/ACE2 axis on experimentally-induced myocardial infarction
(Elsevier Ireland Ltd, 2022-11) Abdel-Nasser, Zeinab M; Zaafan, Mai A; Abdelhamid, Amr M
Myocardial infarction (MI) is a progressive myocardial necrosis that can lead to a number of life- threatening complications. MiRNAs have a crucial role in the pathogenesis of many cardiovascular diseases. Remarkably, miR-122 targets the sirtuin-6 (Sirt-6) gene, which is an essential regulator of cardiovascular function and is considered a partial angiotensin converting enzyme 2 (ACE2) activator. Modulation of this axis is supposed to contribute to MI pathogenesis. The current study aims to investigate the cardioprotective effects of xanthenone through targeting the miR-122/Sirt-6/ACE2 axis on experimentally-induced MI in rats. Xanthenone was administered for 14 days and isoprenaline was injected in the last 2 days of the experiment. Xanthenone treatment resulted in a significant downregulation of miR-122, which further upregulated Sirt-6 and thus activated the adenosine monophosphate-activated protein kinase (AMPK). AMPK increases ACE2 levels and results in a decrease in the level of its substrate angiotensin II resulting in the normalization of the inflammatory cytokines and the cardiac biomarkers. Finally, by targeting the miR-122/Sirt-6/AMPK/ACE2 axis, xanthenone has the potential to be a promising cardioprotective agent against MI.