Browsing by Author "Abdel Razeq, Sawsan A."

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    Densitometric and Ratio Spectra Methods for Simultaneous Determination of Sulfaquinoxaline Sodium and Pyrimethamine in Binary Mixture
    (CAIRO UNIVERSITY, 1/16/2019) El Sanabary, Hoda F.; Fouad, Manal M.; El Demerdash, Asmaa O; Abdel Razeq, Sawsan A.
    Four methods were developed for simultaneous determination of sulfaquinoxaline-Na and pyrimethamine in their combined pharmaceutical formulations. The first one was a densitometric method where chloroform-methanol (9: 1, v/v) was the developing system and the plates were scanned at 254 nm. The obtained spots appeared at Rf 0.64 and 0.35 and determined in the range of 0.5-10.0 µg/spot and 0.1-10.0 µg/spot for sulfaquinoxaline-Na and pyrimethamine, respectively. Furthermore, three spectrophotometric methods manipulating ratio spectra namely, ratio difference method, extended ratio subtraction method coupled with ratio subtraction method and mean centering method were established for the determination of the two studied drugs in the presence of propylene glycol as a solvent. Linear correlation was found over the concentration range of 2-25 µg mL-1 and 3-15 µg mL-1 for the two drugs, respectively. The proposed methods were successfully applied for analyzing the cited drugs in their veterinary pharmaceutical formulations. The obtained results were statistically analyzed and found to be in accordance with those given by a reported method. The validity of the methods was evaluated according to ICH guidelines
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    Densitometric and UV-Spectrophotometric Methods for Simultaneous Determination of Spiramycin adipate in Binary Mixture with Oxytetracycline-HCl or Tetracycline-HCl
    (NLM, 2018) El Sanabary, Hoda F; Fouad, Manal M; Abdel Razeq, Sawsan A.; El Demerdash, Asmaa O.
    Five methods were developed for simultaneous determination of spiramycin adipate and oxytetracycline-HCl and/or tetracycline-HCl in their pharmaceutical formulations. The first one was a densitometric evaluation of thin-layer chromatograms using a mobile phase of methanol: Butanol: Chloroform: Ammonia 1% (5:1:1:1, by volume). The plates were visualized under UV lamp at 254 nm where spots appeared at Rf 0.76, 0.30 and 0.37 for spiramycin adipate, oxytetracycline-HCl and tetracycline-HCl, respectively. The chromatograms of the drugs were measured densitometrically at 240 nm for spiramycin adipate and at 350 nm for both oxytetracycline-HCl and tetracycline-HCl in the range of 0.1-0.8 µg mL-1 and 0.1-1.0 µg mL-1, respectively. Likewise, the simultaneous estimation of the cited drugs was performed by four spectrophotometric methods. Method A was a mean centering (MC) which provided spiramycin adipate determination at 232 nm t oxytetracycline-HCl or tetracyclinea third derivative (3D) through which spiramycin adipate could be estimated at 256 nm while the wavelength 281 nm was selected for oxytetracyclin C was a derivative ratio (1DR) that was established to determine spiramycin adipate only in the presence of oxytetracycline-HCl or tetracycline dual wavelength (IDW) which allowed the determination of oxytetracycline spiramycin adipate and up to 50% of its impurity tetracycline. the concentration range of 5-70 oxytetracycline-HCl and tetracycline to be in accordance with those given by reported methods. The validity of the methods was evaluated according to ICH guidelines.