Browsing by Author "ALY, Amina A"

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    Assessment of irradiated TiO2nanoparticles on the growth and nanoparticles on the growth and nutritional components of broccoli
    (EAP Academic press, 09/02/2021) ALY, Amina A; MARAEI, Rabab W; LOUIS, Yousef A; Safwat, Gehan
    Broccoli is highly tremendously as it is enriched with healthy promoting phytochemicals. This researchwas undertaken to study the feedback of broccoli to different concentrations of un-irradiated and irradiated (50 kGy of gamma rays) titanium dioxide nanoparticles (TiO2NPs). Un-irradiated and irradiated titanium dioxide characterization was accomplished by FT-IR, XRD, TGA, SEM and TEM. Foliar spray of titanium dioxide was applied to the broccoli in regards to the results of the characterizations. The growth traits; plant height, leaves No. per plant as well as stem diameter of plants and physical quality of heads were measured. Also, nutritional components of heads were determined. The results detailed that vegetative growth and physical quality of heads positively responded to foliar application of titanium dioxide compared to those obtained from control and 50 ppm from irradiated titanium dioxide (ITiO2NPs) gave the highest values of all traits. Regarding to nutritional components of heads, the efficiency of photosynthesis increased by using TiO2 (un-irradiated and irradiated) specially, 50 ppm ITiO2NPs. Application of Ti significantly increased the osmolytes concentrations such as proline, total free amino acids and soluble sugars as well as the extracts of heads sprayed with TiO2NPs concentrations displayed a prospective DPPH free radical scavenging action. Likewise, absorption of other nutrient elements and amino acids pool increased by spraying TiO2NPs. It was found that there are several phytochemical constituents identified by GC-MS that contribute to the biological activity of the methanol extract of broccoli heads that were affected by the use of TiO2NPs. It is clear that from above results, the using of ITiO2NPs specially; 50 ppm stimulated growth, resulting in improved quality of broccoli heads which is ultimately reflected in productivity.
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    Physiological variation of irradiated red radish plants and their phylogenic relationship using SCoT and CDDP markers.
    (EAP Academic press,, 09/02/2021) ALY, Amina A; ELIWA, Noha E; BORIK, Zeyad M; Safwat, Gehan
    Greenhouse experiment is carried out to explore the outcome of γ-radiation on physiological and genetic variation in red radish (Raphanus sativus) for two generations. Gamma rays from 60Co were used to penetrate red radish seeds with different dose levels (0.0, 10, 20, 40 and 80 Gy). Plants generated from irradiated seeds and from self-pollination of these plants, called M1 and M2 generations, respectively. Some morphological and physiological traits were then determined, and the genetic diversity of both generations was studied using Start Codon Targeted (SCoT) and Conserved DNA-Derived Polymorphism (CDDP) molecular markers. All studied morphological traits (number of leaves/plants, leave height, root diameter, and root weight) were steadily improved by raising irradiation dose rate, reaching a cumulative raise at the irradiation doe level 40 Gy and decreased at dose level 80 Gy. Photosynthetic pigments of red radish plants released a notable increase by increasing gamma rays dose level for chlorophyll (a), chlorophyll (b) and carotenoids for 40 Gy dose rate. Proline content was elevated proportionally to the irradiation dose level, with the greatest increase seen at dose level of 80 Gy. Moreover, phytochemical screening was detected for the both two generations. Fourteen SCoT primers generated a total number of banding patterns of 194 with average 13.86 and the primer SCoT-33 released the highest number banding patterns (21). The percentage mean of polymorphism for all the SCoT primers was 74.66% and was 66.49 and 63.74% for M1 and M2 respectively. Furthermore, fifteen CDDP primers generated a total number of banding patterns of 186 and the primer CDDP-5 relieved the highestnumber of banding patterns (20). The percentage mean of polymorphism for all the CDDP primers was 73.41% and was 64.38 and 65.91% for M1 and M2 respectively. It could be concluded that gamma irradiation exhibited an appropriate variation in red radish M1 and M2 which was detected by SCoT and CDDP molecular markers