Faculty Of Biotechnology Graduation Project 2017 - 2018

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Browsing Faculty Of Biotechnology Graduation Project 2017 - 2018 by Author "Atef Moaatamed, Shurouk"

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    Studies of the biological activity of cassia fistula.
    (October University for Modern Sciences and Arts, 2018) Atef Moaatamed, Shurouk
    Cancer has been the most dreadful disease from ages till now. However, with the passage of time it been more understood its mechanism, types, some causing factors, and way of its cell duplication. By the progress of science there are now more than one available method for treatment, diagnosis or prevention of cancer. But this progress did not reach till now cheap, fast and effective specific targeting treatment for all types or even one type. For this aim, the field of searching for alternative natural compound extracted from plants to replace those expensive non effective targeting compounds has been wider and advanced. One of familiar plant families is Leguminosae specifically class of Cassia Fistula plant. This type of plant has been examined to ensure its biological activity and by detecting and analysis it show anticancer activity against cell line of liver cancer; hepatocellular carcinoma HepG2. What differ those phytocompounds over commonly used synthetic element is their safety on normal cells. Using brine shrimp eggs and larva was to check the cytotoxicity and cell viability of the compounds that analyzed by GC/MS to identify those phytocompound.C. fistula volatile oil extract was able to inhibit proliferation for HepG2 cancer cell lines at 3.05 ± 0.08μg/ml. The methanolic and oil extracts of Cassia fistula showed good brine shrimp larvicidal activity with lethality concentration (LC50) of 15 and 55 μg/mL, respectively. Finally using GC-MS, the essential oil compounds were identified, whereas39compounds produces and accounted for 90.46% of the total oil, the major compound was compoundsulfurous acid; cyclohexyl-methyl octadecyl ester (21.62%).