Abstract:
Acrylamide is used in the industry and can be a by-product of high-temperature food processing which
has toxic potential in various tissues, and titanium dioxide nanoparticles (TiO2NPs) are widely used in
toothpaste, sweets, food perseveration, chewing gum and medicines. Consequently, humans are daily
exposed to large amounts of acrylamide and TiO2NPs mainly through food intake. However, limited
studies are available on the efect of simultaneously intake of acrylamide and TiO2NPs on the integrity
of genomic DNA and the induction of apoptosis in brain tissues. Therefore, this study estimated the
infuence of acrylamide coadministration on TiO2NPs induced genomic instability and oxidative stress
in the brain tissues of mice. To achieve this, mice were orally administrated acrylamide (3 mg/kg b.w)
or/and TiO2NPs (5 mg/kg b.w) for two successive weeks (5 days per week). The comet assay results
showed that concurrent oral administration of acrylamide and TiO2NPs strongly induced single- and
double stranded DNA breaks, and that the level of reactive oxygen species (ROS) was also highly
elevated within neural cells after simultaneous oral intake of acrylamide and TiO2NPs compared to
those observed after administration of acrylamide or/TiO2NPs alone. Moreover, oral co-administration
of acrylamide with TiO2NPs increased apoptotic DNA damage to neurons by upregulating the
expression levels of P53, TNF-α, IL-6 and Presenillin-1 genes compared to groups administered
TiO2NPs. Therefore, from these results, the present study concluded that coadministration of
acrylamide renders TiO2NPs more genotoxic and motivates apoptotic DNA damage and oxidative
stress induced by TiO2NPs in brain cells, and thus it is recommended to avoid concurrent oral
acrylamide administration with TiO2NPs.
